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    HER-2 expression in cancer cells

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    I'm writing a report on cells which overexpress HER-2. The report consists of 5 mini reports and is for a Biomedical Techniques module I am doing.
    Part of the report is on the monolayer culture and passage of cells and also requires calculations for multiplication rate, doubling time etc (have done these)

    I have two main questions;

    In a monolayer culture would these cells proliferate quicker than non cancerous cells and is the pattern of growth (if plotted) different to that of non cancerous cells? Where can I find info on this - any ideas??

    I have found a report that says the in vitro growth of breast carcinoma cells is limited to one or two passages in a monolayer culture. Why is this? - the report doesn't say, it just goes on to talk about 3D cultures being better.

    I am really struggling to find a way to link writing about the culture and passage of cells, and their doubling rates etc with cells which overexpress HER-2. The report is supposed to focus on techniques used to identify the presence of these cells types so that it can be determined if Herceptin may be a suitable treatment.

    - really feel like giving up as I'm not getting anywhere. Any help would be appreciated.

    Thank you

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    Solution Preview

    The factors that regulate normal cell cycles often go awry in cancer cells. This results in uncontrolled proliferation that leads to tumors that can invade and destroy normal tissue. It is what makes cancer an interesting field of research. Often the gens and proteins that cause cancer are involved in normal development it is only when their expression and occasionally their functions are disrupted that you get a potentially deadly disease.
    The first place to start is to examine what the normal function of HER-2 is. The best place to look for current scientific information is pubmed.com. There are over 9000 scientific articles that contain HER-2. I would suggest starting with some review articles such as:
    -HER-2, TOP2A and Chromosome 17 Alterations in Breast Cancer.
    -HER-2/neu represses the metastasis suppressor RECK via ERK and Sp transcription factors to promote cell invasion. http://www.jbc.org/cgi/content/full/281/8/4718
    -Her-2/neu expression and gene amplification in gastrinomas: correlations with tumor biology, growth, and aggressiveness. ...

    Solution Summary

    Several paragraphs with web references examining the overexpression of HER-2 in cancer cells.