Four Hfr stains of E. coli are known to transfer their genetic material during conjugation in different sequences. Given the time of entry of the markers into the F- recipient:
A. Construct a genetic map that includes all of these markers. (12 points)
B. Label the time distance between adjacent gene pairs. (11 points)
C. Indicate the points at which the F factor is located for each of the strains. (4 points)
Hfr Strain 1 Markers arg thy met thr - -
Times in min 15 21 32 48 - -
Hfr Strain 2 Markers mal met thi thr try -
Times in min: 10 17 22 33 57 -
Hfr Strain 3 Markers phe his bio azi thr thi
Times in min 6 11 33 48 49 60
Hfr Strain 4 Markers his phe arg mal - -
Times in min 18 23 35 45 - -
A. Here is the genetic map including all the markers (drawn on the basis of distance between the entry of markers into the F- recipient).
(fig 1 in attachment)
Here is the distribution of markers on the chromosome along with the time difference (in minutes) between different markers. (fig 2 in attachment)
B. The ...