Explore BrainMass

Explore BrainMass

    Plasmid mapping: Example problem

    This content was COPIED from BrainMass.com - View the original, and get the already-completed solution here!

    A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Draw a plasmid map.

    © BrainMass Inc. brainmass.com December 15, 2020, 2:04 pm ad1c9bdddf

    Solution Preview

    I won't draw the plasmid map for you, because it's so easy to do. Instead, I'll give you step by step instructions on how to discover it for yourself.

    Here we go.

    First of all, you know that a plasmid is circular. So draw a circle.

    When we digest the plasmid with BamHI, we end up with two fragments. How can we get two linear pieces from a circle?

    We must have two cut sites. Think of it this way. A restriction enzyme is just like a pair of scissors. It cuts the circle. So, if ...

    Solution Summary

    Discusses plasmid mapping.