Explains why pro-insulin can be refolded but not insulin (following Anifansen's experiments with ribonuclease)© BrainMass Inc. brainmass.com June 3, 2020, 5:43 pm ad1c9bdddf
In Anfinsen's original experiment, he denatured ribonuclease with 8M urea (this is a dissociating solvent and breaks all weak bonds). 2-mercaptoethanol is a reducing agent and reduces disulphide bridges to SH groups (sulphydryl), thus breaking them. This converts the enzyme to a random ...
The solution explains why pro-insulin can be refolded but not insulin. The Anifansen's experiments with ribonuclease are given.