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    Difference in folding characteristics of Pro-Insulin and Insulin

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    Explains why pro-insulin can be refolded but not insulin (following Anifansen's experiments with ribonuclease)

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    https://brainmass.com/biology/biological-chemistry/difference-folding-characteristics-pro-insulin-insulin-36195

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    In Anfinsen's original experiment, he denatured ribonuclease with 8M urea (this is a dissociating solvent and breaks all weak bonds). 2-mercaptoethanol is a reducing agent and reduces disulphide bridges to SH groups (sulphydryl), thus breaking them. This converts the enzyme to a random ...

    Solution Summary

    The solution explains why pro-insulin can be refolded but not insulin. The Anifansen's experiments with ribonuclease are given.

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