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Difference in folding characteristics of Pro-Insulin and Insulin

Explains why pro-insulin can be refolded but not insulin (following Anifansen's experiments with ribonuclease)

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In Anfinsen's original experiment, he denatured ribonuclease with 8M urea (this is a dissociating solvent and breaks all weak bonds). 2-mercaptoethanol is a reducing agent and reduces disulphide bridges to SH groups (sulphydryl), thus breaking them. This converts the enzyme to a random ...