A solution has a concentration of 3.8mg/ml of protein.
a) 7 μl of a 10-5 dilution of the original solution contains how much protein?
b) How much should you dilute the original solution to get a protein concentration of 2 μg/ml?
If you could also give me a web site or attachment that may help me to under stand other problems like this i would apprecate it. Thank you.
Solution Preview
I'm going to start with the (b) part:
<br>To figure out how much you need to dilute a 3.8 mg/ml solution to obtain a 2 ug/mL solution, you simply divide the original concentration by the final, desired (more dilute) concentration. But first, you have to make the units of concentration agree.
<br>There are 1000 micrograms (ug) in a milligram (mg),
<br>so 3.8 mg/mL = 3800 ug/mL
<br>
<br>(3800 ug/mL) / (2 ug/mL) = 1900
<br>
<br>So ...
... volumetric flask 1X10-mL volumetric flask pipette filler bulbs ... voltage for each of the six dilute solutions ... Aspirin 1. The voltage of diluted solution prepared ...
... In fact, if we dilute the solution by a factor ... by what factor should the stock solution be diluted? ... the standard solution using a volumetric pipet and transfer ...
... 2) Calculate the final concentration when the 5mM is diluted to 1 ... Therefore, we can use a burette (or pipette) to obtain ... Second part: If you dilute the 5 mM to 1 ...
... the reaction flask, it will have no impact because the moles are based on the measurement of a dry pipette. Water in the reaction flask will dilute the solution ...
... Into tubes C1 and C2, pipette 0.5 cm3 of a 1 in 100 dilution of supernatants C1 and C2. Into tubes F1 and F2, do the same for supernatants F1 and F2. ...
... in acid and transferring to a 250-mL flask and diluting to volume ... are analyzed by transferring 50-mL portions with a pipet to 500 ... We need to back dilute (undilute ...
... Therefore, the initial blood sample was diluted 1:20. ... we use this number and back-calculate the dilution factor of ... by the glucose oxidase method Pipette 0.5cm3 ...
... 2. Using a 1-ml pipette, transfer 0.574ml of ... a concentrated stock solution and yields progressively dilute solutions ... 1.0g/ml stock is diluted with dH2O to give ...
... The solution is 0.2 M. 3. Dissolve 3.4 g of NaH2PO4 (or KH2PO4 ) in water and dilute to 100 mL. ...Pipet 1.00 mL of the bromothymol blue solution into each flask. ...
... Load 10µL of marker/buffer mix) Caution: Inserting the pipette tip too far into the ...Dilute the protein sample by at least 1:1 with the sample buffer, and then ...
