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Phosphoric Acid, Iron in Serum, and pH of a Mixture

1.In the laboratory you have prepared various reagents for your experiments. briefly describe the preparation of 3.00 L of 0.150 M H3PO4 from concentrated commercial reagent. This reagent if 86.0% H3PO4 and has a density of 1.84g/mL. The molar mass of H3PO4 is 97.995 g/mol

2. The following data were obtained for the determination of iron in serum by a spectrophotometeic method. Assuming the validity of Beer's Law for the experiment calcualte the concentration of iron in serum..
Sample Absorbance at 562 nm in 1.000 cm cell
Blank 0.038
3.00 µg Fe standard 0.329
Serum sample 0.219

3.What pH do you expect if you mix 25.mL of 0.2M NaOH with 50.0 mL of 0.15 M HNO3 and then dilute to 250.0mL in a volumetric flask?

Solution Summary

This solution explains:

1) How to prepare a solution of phosphoric acid.

2) How to calculate the concentration of iron in serum.

3) How to calculate the pH of a mixture of acid and base solutions.

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