Understanding dilution factors
Question: A courier has just delivered a 2.0 mL sample of serum to your laboratory for analysis. In your analysis, you will combine a 0.05 mL aliquot of the serum with 9.95 mL of phosphate buffered saline diluent. Calculate the dilution factor.© BrainMass Inc. brainmass.com October 10, 2019, 6:41 am ad1c9bdddf
Before answering the question, let's define some terminology.
- Aliquot = a measured sup-portion (or sub-volume) of an original sample or solution
- Diluent = a material (in this case, phosphate buffered saline, also referred to as "PBS") that is used to dilute a sample
- Dilution factor (DF) = a ratio of the final volume to the aliquot volume
Next, let's look at the information provided in the question:
- Volume of blood = 2.0 mL
- Aliquot size = 0.05 mL
- Diluent volume = 9.95 ...
A question and solution to help students understand and calculate dilution factors for chemistry, biochemistry, biology, or biomedical applications.