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Enterobacteriaceae

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The family Enterobacteriaceae contains some organisms living in the intestines without harming the host and some organisms that are harmful to the host.

Based on your research, respond to the following:

What is meant by the term "enteric pathogen"?
Why are anaerobic organisms generally not seen in a routine fecal specimen or culture?
What are the indole test, methyl red test, voges-proskauer test and citrate test (IMViC) reactions? Describe in detail all four reactions (what media is used, important ingredients, what each reaction measures, and what positive and negative results mean).
Create a flowchart for the isolation and identification of specific enteric bacteria from fecal samples.

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Solution Preview

Based on your research, respond to the following:

What is meant by the term "enteric pathogen"?

Enteric pathogens are gastrointestinal organisms spread by contamination of foods mainly of animal origin and among people who may be carriers. (1)
Some examples of invasive enteric pathogens are the Salmonella or Shigella genera infection by invading the intestine. One enteric pathogen Salmonella cacan translocate across the mucosa of the small intestine and cause systemic disease, such as localized inflammator response in areas of infection. (2)
Another enteric pathogen Shigella can cause diarrheal episodes. (2)

Why are anaerobic organisms generally not seen in a routine fecal specimen or culture?
This question has it the other way around. Aerobic organisms are typically not seen in routine fecal speciment because most microbes of the feces are strict anaerobes. The stool or fecal culture usually contain microbes such as Campylobacter, E. coi (057:H7), Shigella, and Salmonella. These microbes are usually strict anaerobes. There are more than "99% of the intestinal microflora consists of obligate and facultative anaerobes." (3) The isolation and cultivation of the intestinal microflora used strict anaerobic technique to count and identify the microbes in the ...

Solution Summary

The solution discusses the family of Enterobacteriaceae and answers questions based on the relative research.

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enteric pathogens

Explain what is meant by an enteric pathogen as opposed to enteric bacilli.

Explain why anaerobic organisms are generally not the enteric pathogens seen in a routine fecal specimen. Why are these anaerobic organisms not seen in a routine fecal culture?

What happens when enteric pathogens are seen in fecal specimens?

Explain the principle and advantage of using a rapid identification system, such as the Enterotube. How would you rate the Enterotube system with conventional bacteriological procedures to identify members of the Enterobacteriaceae family? Why?

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