Single Substitution Polymorphism
Not what you're looking for?
5'tggtggtggaggctggggtcaaggtggtagccacagtcagtggaacaagcccagtaagccaaaaccaacaygaagcatgtggcaggagctgctgcagctggagcagtggtagggggccttggtggctacat3'
Can this sequence be cut by a restriction enzyme?
Can the fragments and coupled with electrophoresis be used to find a single substitution polymorphism?
Purchase this Solution
Solution Summary
Find an appropriate restriction enzyme that will cut the given sequence. The solution details how you would go about finding the restriction enzyme. The solution then tells you how, armed with that information how you can go about finding a single substitution polymorphism within that DNA sequence. Some background is provided to explain the answer and help you answer similar questions.
Solution Preview
The sequence you mentioned can be cut by a number of restriction enzymes (REs). I find this tool to be very helpful in picking REs: http://tools.neb.com/REBsites/index.php
You basically paste in your sequence and it will tell you the different REs and can generate a virtual gel electrophoresis. In your case there are 49 different REs that can digest your sequence ...
Purchase this Solution
Free BrainMass Quizzes
Hemophilia: Fact or Fiction
Do you know the truth about hemophilia? Test your knowledge here.
Basic Concepts in Neuroscience
This quiz provides a review of the basic concepts in neuroscience.
Breastfeeding Basics
How much do you know about breastfeeding? Find out with this quiz!
The Plant Body
This quiz will test your knowledge of the anatomy of a common plant.
BioChemistry Basics
This Quiz will test your knowledge of the amino acids used in biological systems