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DNA-Pyrosequencing

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Show, with examples, how the 'pyrosequencing' method has been useful for very high throughput applications.

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Solution Summary

Examples of pyrosequencing are discussed and references to the primary literature are provided.

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Hepatitis C virus (HCV) typing:

For HCV typing semi-conserved regions such as NS5 or 5′ untranslated region have been used as a target for subtyping. Pyrosequencing of the region could exactly determine all possible subtypes. For population-specific subtyping, multiplex pyrosequencing could ...

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