Determining paternity
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Please state whose daughter it is? How did you come up with that conclusion?
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Solution Summary
Detailed answer explaining how paternity can be established via VNTR analysis Web references provided.
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This problem uses VNTR (variable number tandem repeats) to establish paternity. Because of the variable nature of VNTRs (i.e. the copy number can be different from unrelated persons) a panel of them can be used to suggest family relation. While unrelated individuals may have the same VNTR at one loci the probability of a coincidental match decreased with the number of locus examined. In this manner a panel of VNTRs can act like a fingerprint. Because they are inherited the VNTRs will be a mixture of the VNTRs found in both parents. To examine these VNTRs you can perform RFLP (restriction fragment length polymorphism) where by you digest genomic DNA with a specific restriction enzyme and examine the different fragment lengths on a gel. With the advent of PCR and the availability of genome sequence PCR primers can be designed for specific VNTR loci. They can then be amplified by PCR and examined of a gel. The length of the PCR fragment will indicate the size of the repeat. By comparing a subjects pattern with those of potential parents relations can be established.
In this problem we are given the VNTR pattern for a claimed child, Liliana Pereya (#9), of Monica Grinspon and Claudio Ernesto Logares. We also have the VNTR pattern for a ...
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