The autoradiogram below shows the genomic DNA fragments produced by digesting genomic DNA with the restriction enzyme Mst II and probing the membrane with a DNA probe specific for the beta globin chain gene of hemoglobin. The parents are C and D, and the children are E and F. Hb S indicates a mutated gene leading to the sickle cell condition. Hb A indicates normal hemoglobin.
Please see attachment (take note: HbS goes with first row; HbS with second row)
a) Does child F have sickle cell anemia
b) Why does child E have only one band showing on the gel?
A. Yes child F does have the mutated gene that leads to sickle cell anemia. ...
This solution contains an overview of the autoradiogram of a child.