Propose an experiment to distinguish between semi-conservative, conservative, and dispersive synthesis after a single round of replication.© BrainMass Inc. brainmass.com March 21, 2019, 10:12 pm ad1c9bdddf
The faithful transmission of hereditary information depends on accurate replication of the genetic material.
E. coli cells should be cultured in a medium containing heavy isotope of nitrogen (N15 rather than normal nitrogen) and it should be free of contaminants. This isotope is inserted into nitrogen bases which then incorporates into newly synthesized DNA strands. After two or three cell divisions, the DNA of the cells will be labelled with heavy isotope. The cells should be carefully remove from the heavy nitrogen medium and ...
This solution discusses the differences between semi-conservative, conservative, and dispersive synthesis after a single round of replication in 329 words.