Tetrazolium test for determining viability of seeds

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Objective:
Seeds form the most important, cheapest component and basic input of mass plant cultivation. Only seeds with assured quality will respond to fertilizers, water, pesticides and other inputs and pay required dividends. Progress in food production is assured only when good quality seeds can be identified, multiplied and marketed efficiently. The performance of a seed can be determined by studying it germination and viability potential. One of the most significant advances in seed testing technology in recent years is the topographical tetrazolium method. It's the fastest test available in testing seed viability for both dormant and non-dormant seeds, which is a boon to the seed industry for quicker decision making. The test provides for a rapid evaluation of seed lot in a short time of 2-24 hrs.

<br>Requirements:
<br>Petridishes, Scalpel, Forceps, Magnifying lens or Stereoscopic microscope, Dispensing bottle, Distilled water, Filter paper, Oven or incubator to maintain temperature at 40-45 degree centigrade.

<br>Reagents -
<br>1. Tetrazolium solution: The concentration of the solution varies according to the ...