Energy Absorbance

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  Both chloramphenicol and cycloheximide can have an effect on the redistribution of energy between the two photosystems in chloroplasts. This effect may influence an increase in nitrate reductase activity. Reference 1.

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  By energy conservation law, Reflectance + Transmittance + Absorbance = 1. We have assumed the absorbance to be zero for this isotropic medium since that factor is always less. Hence the conservation of energy is valid here.

• To calculate protein concentration with given absorbance

  Equation for the value given is : y = 0.122x Mean of the given absorbance : (.31 + .318 + .322)/3 = 0.316 Protein content for this absorbance : 0.316 = 0.122 x = 2.59 mg / cm3 Mean of the given absorbance : (0.444 + 0.423 + 0.443) / 3 = 0.436

• Solution Concentration Calculations

  B.Record these results in your Excel® file with the average Absorbance of these results. C.Calculate the average absorbance and use this value for an absorbance point on your graph.

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  299249 Spectrophotmetry Measured Absorbance 1.) The measured absorbance of a sample in a spectrophotometer is 0.704. What percentage of the incident light is being transmitted? 2.)