1) How would you graph to show how the rate of reaction would be affected by the presence of a competitive inhibitor?
2) How would you graph to show how the rate of reaction would be affected by the presence of a non-competitive inhibitor?
If you make a "Lineweaver-Burke" plot you can easily tell the difference between a competitive and a non-competitive inhibitor. A Lineweaver-Burke plot is also called a "double reciprocal" plot of velocity (rate) vs. substrate concentration. Therefore, it's a plot of 1/velocity vs. 1/[S].
When you plot your kinetic data like this, you will produce a straight line curve. The slope will be Km/Vmax. The x-intercept will be -1/Km and the y-intercept will be ...
This solution provides explanations for problems related to competitive and non-competitive inhibitors