How many 100-W lightbulbs connected to 120V can be used without blowing a 15-A fuse?
I = P/V
I = 100W/120V = .8A
I divided 15 A by .8 A to equal 18.75; therefore, you could use 18 bulbs.
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This solution provides an explanation regarding lightbulbs and fuses.
Gene Expression Systems are figured.
Let's assume that I fould a valuable gene fragment (ex. DNA sequence homology with one of the human myosin II genes)
1. Before I proceed for therapeutic development, How would I know if this gene is actually expressed in humans? What method?
2. Now let's assume it is expressed in human. I wish to express the cDNA corresponding to this gene in order to determine its function. How would you construct an expression vector? Would you use the genomic fragment or a cDNA?
3. How would you transfect your expression plasmid into cells, and what type(s) of cell might you use?
4. How would you confirm expression of your construct and encoded protein? (functions and methods)
These questions are pondered.View Full Posting Details