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Lab Practical: Factors Affecting Enzyme Activity

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Determination of optimum pH:
Urease has an optimum pH, depending on the source of the enzyme, determine this value from the graph plotted.

Determination of the effect of substrate concentration:
Urease has an optimum pH, depending on the source of the enzyme, determine this value from the graph plotted.

Determining the effect of temperature on enzyme activity:
Calculate the Q10 for the reactions at l5C and 25°C.

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This solution identifies problems with some of the results, provides extra explanation, and provides calculations and plots. There are two attachments, one Word document and one Excel spreadsheet with graphs and data.

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Note: This is a common misconception. I surveyed a large number of human enzymes and found a wide spread of optimum temperatures, from 20 °C to 80 °C. Changing an enzyme's optimum temperature is not easy, and there is no evolutionary advantage to having every enzyme working optimally at 37 °C, so long as the enzymes ...

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