Understanding Interpolation and Extrapolation for Assays
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This article describes the difference between interpolation and extrapolation in the context of the Bradford protein assay, and why you cannot extrapolate the concentration.
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Solution Summary
This article explains the difference between interpolation and extrapolation in the context of the Bradford protein assay and why extrapolation is not possible. It also describes some rules highlighting what to do with your sample results within this context.
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For a Bradford assay (or MANY other assays) that uses a standard curve, it is not appropriate to extrapolate outside of the range of your "standards". In this assay, you use a set of "standards" of known protein concentration in order to define the relationship between concentration of protein and absorbance value for a given assay run. A standard curve is performed every time the assay is run because small changes in temperature, incubation time, etc., might change the relationship between concentration of protein and absorbance value during that particular run. In other words, if you ran an assay last week, and are running it again this ...
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