Categorize each as a determinate or an indeterminate error, and further categorize determinate errors as instrumental, operative, or methodic; (a) An unknown being weighed is hygroscopic; (b) One component of a mixture being analyzed quantitatively by
The separation is achieved by the distribution of the mixture between two phases: one that is stationary and one that is mobile.
Typical Set-Up Injector - This is a fully automated component that loads your mixture onto the column. Retention Time - This is the time it takes for an individual component to pass through the column.
One length of solvent will take the compound halfway down the column. A second length of solvent should theoretically take it the rest of the way. -Explain why or why not NaCl can be purified by column chromatography.
The principle of GC/MS requires a sample solution to be injected into the gas chromatography (GC) inlet where it is vaporized. The carrier gas then moves the vapor onto a chromatographic column.
The desired result is that each component of the deposited mixture is moved a different distance up the plate by the solvent. The components then appear as a series of spots at different locations up the plate.
604844 The Interpretation of Gas Chromatograms A chromatogram would feature lines instead of peaks if: a) the best equipment available is used b) the separation is done by capillary electrophoresis c) if the temperature nears (or is equal to) the absolute
It is often found that by controlling the capacity factor, k', separations can be greatly improved. This can be achieved by changing the temperature (in Gas Chromatography) or the composition of the mobile phase (in Liquid Chromatography).
The one with the higher vapor pressure or the one with the lower vapor pressure? The one with the higher vapor pressure boils first, because more of it is in vapor form to begin with.
The target protein is retained on the column, then removed by rinsing the column with a salt solution or other agents.