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Table 1. |
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Influence
of substrate concentration on enzyme activity |
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Concentrations Amounts
microliters |
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Reagents |
1 |
2 |
3 |
4 |
5 |
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L-tyrosine |
20 |
40 |
60 |
80 |
100 |
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Tyrosine |
20 |
20 |
20 |
20 |
20 |
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Phospate Buffer |
860 |
840 |
820 |
800 |
780 |
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Activity After 2mins |
0.074 |
0.250 |
0.266 |
0.291 |
0.184 |
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Activity After 4mins |
0.178 |
0.336 |
0.498 |
0.469 |
0.429 |
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Table 2. |
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Influence of enzyme
concentration on activity |
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Concentrations Amounts
microliters |
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Reagents |
1 |
2 |
3 |
4 |
5 |
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L-tyrosine |
100 |
100 |
100 |
100 |
100 |
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Tyrosine |
10 |
20 |
30 |
40 |
50 |
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Phospate Buffer |
790 |
780 |
770 |
760 |
750 |
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Activity After 2mins |
0.133 |
0.215 |
0.264 |
0.560 |
0.431 |
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Activity After 4mins |
0.355 |
0.497 |
0.455 |
0.531 |
0.522 |
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