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FC assay

In the Folin Ciocalteu assay, why do we need to excess FCR? Also give 3 reasons why gallic acid is used as standard for the total phenolic assay?

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FCR is taken in excess because it doesn't reduce only phenolic compounds but a number of others including thiols, carbohydrates, amino acids, ketones and other non-phenolic compounds. Hence more FCR than what would be necessary ...

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