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Why scientists purify proteins; principle of size exclusion

Why do scientists want to purify proteins? How does size exclusion chromatography works?

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Why do scientists want to purify proteins?
Scientists in biotechnology must face the challenge of protein purification regularly. But the question arises, why do scientists need to take the pain? In a research environment, proteins must be purified in order to determine their structure and study their biochemical properties. In industrial settings, proteins are purified on a larger scale in order to be sold as products such as drugs, vaccines, ...

Solution Summary

Scientists in biotechnology must face the challenge of protein purification regularly. But the question arises, why do scientists need to take the pain? In a research environment, proteins must be purified in order to determine their structure and study their biochemical properties.

Size exclusion chromatography or gel filtration chromatography works on the principle of separation of proteins on the basis of their size.

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