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Determine the primary structure of the peptide

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In trying to deduce a primary structure of an peptide sequence...

When does a polypeptide NOT contain a free carboxyl group? Are all peptides linear (or can they have a cyclic C-terminus)?

What happens when treatment of the intact peptide with 2,4-dinitroflourobenzene (followed by complete hydrolysis and chromatography) yields an amino acid derivitive the involves the amino group of the side chain rather than the alpha-amino group?? Does this matter to the structure of the primary sequence?

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Question: When does a polypeptide NOT contain a free carboxyl group? Are all peptides linear (or can they have a cyclic C-terminus)?

Response: Yes, some small peptides can be cyclic, meaning that the C-terminus is linked to the N-terminus via a ...

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