How do I predict, using the Michaelis-Menten equation, the volume of oxygen collected?
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Aim:
I am investigating the changes of hydrogen peroxide concentration on the rate of decomposition of catalase.
Outline of method:
The apparatus involve yeast(catalase), hydrogen peroxide, a 100cm3 gas syringe to collect oxygen. I am calculating the volume of gas collected in 30 seconds. The concentrations I plan to do are 0.1M, 0.5M, 1M, 2M, 4M. The concentration of the yeast is 10g of bakers yeast granules dissolved in 100g of water.
How do I predict, using the Michaelis equation, the volume of oxygen collected for the 5 given concentrations? in order which to make a prediction of the graph which should form a hyperbolic curve. Please could you give an example of the complete working out for any given concentration, where necessary (if required) making up values of any data I omitted.
Could you also explain how the units of concentration (Vol, % and M) relate to one another?
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Solution Summary
The expert determines how do I predict the changes using the Michaelis-Menten equation. The changes of hydrogen peroxide concentration on the rate of decomposition of catalase are given.
Solution Preview
We need the effect of H2O2 concentration on the rate of the decomposition of hydrogen peroxide when catalyzed by the enzyme catalase. Catalase is one of the fastest acting enzymes known. It decomposes hydrogen peroxide to oxygen and water. One molecule of catalase can decompose 40000 molecules of H2O2 per second at 0o C and can produce 1012 molecules of Oxygen per second. The chemical reaction-taking place is
2 H2O2 + Enzyme ----- > 2 H2O + O2 + Enzyme
If there are more molecules, since the chances of collision increases, the rate of the ...
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