In recent years, molecular biology has undergone a revolution that can be partially attributed to the advent of easy DNA sequencing methods (thanks to Fred Sanger's work published in 1977), and yet the sequencing technology continues to change. Briefly discuss new developments in DNA sequencing technology.
Since the advent of the Sanger method in 1977, many changes have taken place. The major ones are briefly discussed below:
a) The first big revolution came with the advent of PCR (Polymerase Chain Reaction). Sanger's original method required the subcloning of a sequence of interest into an M13 vector to produce many copies of ssDNA. M13 was a difficult organism to grow and ...