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Oligomycin Inhibition: Mechanism And Effect On Metabolism

The addition of oligomycin to mitochrondria markedly drecreased both the rate of electron transfer from NADH to oxygen and the rate of ATP formation. The susbequent addition of DNP leads to an increase in the rate of electron transfer without changing the rate of ATP formation. What does oligomycin inhibit and what is the mechanism of inhibition? (Be detailed with your explanation)

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Addition of oligomycin decreases electron transport and oxidative phosphorylation. However, when DNP is added, ATP production is still inhibited but electron transport increases. What's going on? Since we know that DNP is a proton ionophore, it works at decoupling electron transport with oxidative phosphorylation. That is to say that DNP binds H+ and moves them across the mitochondrial membrane where otherwise the only other route across is through the ATP synthetase enzymes. Therefore, it should be clear ...

Solution Summary

Detailed explanation of oligoymycin's role in the cell.

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