The diazo compound O-(2-diazoacetyl)-L-serine is a powerful inhibitor of glutamine amidotransferases. If growing cells are treated with this compound, what intermediates of nucleotide biosynthesis would accumulate. Please explain your answer.
A large group of biosynthetic enzymes are able to catalyze the removal of the ammonia group from glutamine and then to transfer this group to a substrate to form a new carbon-nitrogen group. This catalytic activity is known as Glutamine amidotransferase (GATase).
Glutamine amidotransferases (GATases) transfer the glutamine amide nitrogen to variety of substrates. Most GATases can use ammonia (NH3) as an alternative nitrogen source. Sites for ...
This solution provides information the intermediates accumulating in presence of inhibitor diazo compound O-(2-diazoacetyl)-L-serine which is a powerful inhibitor of glutamine amidotransferases.