Hematology II Test Review

1. After staining a peripheral smear with wrights stain and a buffer with the proper pH, the RBCs appear pale pink and the WBCs stain very weakly with little or no detail. One possible explanation is

2. only 2.0 ml of blood is added to a 7.0 ml draw Na EDTA (powdered) tube. Which of the following test results would be expected if it is used

falsely lowered RBC
falsely elevated Hemoglobin
falsely decreased hematocrit
normal ESR

3. The following values were obtained over the first ten days of control for a leukocyte determination using a particle counter: 1-7.0 2. 7.1 3. 6.9 4. 6.5 5. 6.7 6. 6.9 7. 6.6 8. 6.7 9. 6.5 10. 6.4 The coefficient of variation for the analyte is 3.5% . Mean is 6.95 Evaluation of the statistical pattern and the coeficient of variation indicated

4. While performing a WBC differential on a finger stick prep smear, no platelets were observed, yet on the printout from the analyzer, a count of 180,000 was obtained. What would you do?

report no platelets seen
report the findings to the supervisor immediately
request a venous sample
look at the edges and trailers for platelet clumping

5. a hemoglobin of 12.5 correlates best with a hematocrit of
37.5

6. A properly calibrated and controlled instrument that uses the principal of electronic empedence produces repeated (x3) values on a blood sample: RBC 4.01 x 10 12/L HCT 32 % HGB 12.0 MCV 80 MCH 32 MCHC 37.5 The most likely explanation for these results is:

iron deficiency anemia
hereditary spherocytosis
high titer of cold agglutinins
high reticulocyte count

7. Using an undiluted CSF specimen, 150 WBCs are counted in the four large corner squares on one side of the hemocytometer. What is the total white count?

38/mm3
375/mm3
750/mm3
3750/mm3

8. The following is an indicator of random error ( imprecision)

method compasrison using linear regression to calculate cefficient of correlation
repretitive sampling of the same specimen and calculating coeeficient of variation
linearity study
calibration

9. You are a CLS evaluating the results from a patient. The RBC count is low in the face of a normal hemoglobin and hematocrit. ( rule of threes does not fit) On the smear, you notice agglutinated RBCs. What would you do?

10. In the peripheral examination of the smear at 900 to 1000x a total of 100 platelets was counted in 5 fields. What is the closest estimate for the total Platelet count? All answers are in platelets/microliter. Normal range is 140,-480,000

11 Examination of a wright stained smear revealed many smudge cells with very few intact leukocytes. What is the appropriate corrective action to minimize the number of smucge cells?

12. A CLS noted a significantly decreased platelet count on a patient. In examining the peripheral smear platelets were seen to adhere to the neutrophils. What if any remedy is available for this problem?

recollect in citrate ( same as for platelet clumping) and rerun the machine count

allow the specimen to sit for 5 hours at 25 degrees and re analyze

warm the specimen to 37 degrees F for 15 minutes and re-analyze

no correcftive action is available