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Designing of protocol to prepare dilution series

A culture was determined to have 5.4 x 10^6 CFU/ml. Design a stepwise protocol to determine this number. For practice use different volumes of diluent and diluate.

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The dilution series was prepared using water blanks and the dilutions were palted on appropriate media at required temperature. Number of colonies were counted after incubation. For details, please see the attached file.

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