Determining Activation Energy from Rate Constant for an Enzyme-Catalyzed Reaction
Not what you're looking for?
Here is the problem I have to solve:
If 10 g of pure carbonic anhydrase catalyzes the hydration of 0.30 g of carbon dioxide in 1 min at 37 degrees C under optimal conditions, what is the turnover number (kcat) of carbonic anhydrase (in units of min-1)?
I have already calculated the turnover number, which is 2.0 x 107 min-1, but I'm kind of lost with two other questions.
(b) From the answer in (a) calculate the activation energy of the enzyme catalyzed reaction (in kJ/mol)
.
(c) If carbonic anhydrase provides a rate enhancement of 107, what is the activation energy for the uncatalyzed reaction?
Purchase this Solution
Solution Summary
This solution shows step-by-step calculations to determine the activation energy of the reaction with carbonic anhydrase and also the activation energy of the uncatalyzed reaction. Brief explanations are also included.
Solution Preview
Please see attached file
From transition-state theory, an expression can be derived that relates the magnitude of a rate constant (k) to the activation energy:
k = (kT/h)e-G/RT
where k is the Boltzmann constant and h is Planck's constant. In this case, G is the activation energy ...
Purchase this Solution
Free BrainMass Quizzes
Light and Sight Vocabulary
This quiz introduces basic definitions of vocabulary related to light and how human eyes. This information is important for an understanding of sight.
Pregnancy Knowledge
How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!
Breastfeeding Basics
How much do you know about breastfeeding? Find out with this quiz!
The Heart
This quiz test the understanding of the heart and some of its parts. It is important to understand how the heart functions and what makes it function.
Birth 101
Do you know about childbirth? Find out with this quiz.