Purchase Solution

In vitro translation and the production of active proteins

Not what you're looking for?

Ask Custom Question

Please help with the following problem.

Knowing the sequence of a protein, a scientist synthesizes the corresponding RNA. Using the machinery of a cell and this RNA, the scientist then resynthesizes the protein. Should the scientist expect to recovery an active protein from this experiment?

Purchase this Solution
Solution Summary

In this solution the question regarding whether in vitro translated protein can be expected to be active when purified is considered and answered, covering numerous perspectives related to molecular biology and biochemistry. It discusses whether the scientist should expect to recover an active protein from this experiment. The explanation is given in 190 words.

Solution Preview

Problem: Knowing the sequence of a protein, a scientist synthesizes the corresponding RNA. Using the machinery of a cell and this RNA, the scientist then resynthesizes the protein. Should the scientist expect to recovery an active protein from this experiment?

Solution:
The answer to this question depends heavily on the nature of the protein ...

Purchase this Solution
Free BrainMass Quizzes
Human Anatomy- Reproductive System

Do you know your reproductive anatomy?

Do You Know Your Macromolecules?

This quiz will assess your knowledge of the macromolecules that are important to living things.

BioChemistry Basics

This Quiz will test your knowledge of the amino acids used in biological systems

Breastfeeding Basics

How much do you know about breastfeeding? Find out with this quiz!

Comfort Measures For Labor

Are you ready to doula someone through labor?

View More Free Quizzes
Related BrainMass Solutions

  • Enzymatically Recombinant cIII Deacytylases

    Expression of recombinant proteins in Escherichia coli in an enzymatically active form. Mammalian cell culture systems •This method allows the purification of enzymatically active sirtuins directly from mammalian cells.

  • Western blot analysis

    From what you know about the signal hypothesis, why does the size of the protein in lanes A and B stay the same in vivo and in vitro? Why does the size of the protein in lanes C and D differ in vivo and in vitro?

  • Protein Synthesis: Transcription and Translation

    chain synthesis and add ribonucleotides to the chain Transcription factors: proteins required to help RNApol find/bind the initiation site (several in prokaryotes, dozens in eukaryotes) In prokaryotes, the protein can be made directly off of the

  • Draw and label an in vitro cellular aging curve-Phase

    Comparison: Transformation In vitro aging In vitro process but here uptake of DNA or RNA is taking place by the cell This is also an in vitro process but here cells are indefinitely growing in the in vitro medium.

  • Protein Synthesis and the Effects of Treating a Cell with RNAase

    of amino acids in protein synthesis rRNA = ribosomal, structure + catalysis of protein synthesis Genes are perpetuated as sequences of nuclei acids [expressed in the form of proteins] by way of replication, transcription and translation.

  • Transcription and Translation

    Most of the data on the control of elongation has been derived from in vitro model systems where the rate of elongation is considerably slower than it is in vivo (Ucker and Yamamoto, 1984).

  • An essay about transcription in eukaryotes.

    The transcription process involves numerous proteins that act in a concerted fashion to allow RNA polymerase to work. These proteins are all subject to regulation and are points of control in the transcription of a gene.

  • Diagram of DNA transcription through RNA translation.

    478059 The process of protein production A diagram of the process of protein production beginning with transcription of DNA and ending with the production of a polypeptide (protein) from RNA translation at the ribosome.

  • mRNA Stability and Translation

    sequester it in regions of high or low activity) The expert names five features of mRNA that can regulate its stability or translation.

View More Related Solutions