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Restriction sites

You are attempting to map the restriction sites for Ecor I and BamH I on a circular DNA molecule. After conducting single digests with Ecor I and BamH I as well as a double digest, you obtain the following data:

Ecor I BamH I Ecor I + BamH I

fragment 1 8000bp 2500 bp 1500 bp
fragment 2 5500 bp 2500 bp
fragment 3 4000 bp

What is the total length of the uncut DNA molecule?

How many restriction sites are there for BamH I?

Draw a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.

Solution Preview

Since EcoRI gives a single fragment of 8000 bp we know that there is only 1 site for EcoRI. Therefore, the total length of the DNA molecule is 8000 bp. We can also ...

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