You are attempting to map the restriction sites for Ecor I and BamH I on a circular DNA molecule. After conducting single digests with Ecor I and BamH I as well as a double digest, you obtain the following data:
Ecor I BamH I Ecor I + BamH I
fragment 1 8000bp 2500 bp 1500 bp
fragment 2 5500 bp 2500 bp
fragment 3 4000 bp
What is the total length of the uncut DNA molecule?
How many restriction sites are there for BamH I?
Draw a diagram of the circular DNA molecule, showing the locations of the restriction sites for Ecor I and BamH I , as well as distances between them.
Since EcoRI gives a single fragment of 8000 bp we know that there is only 1 site for EcoRI. Therefore, the total length of the DNA molecule is 8000 bp. We can also ...