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Restriction Enzyme DNA

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The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces.Indicate wheter DNA ligase can join the following pieces of DNA.
a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .

b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.

c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.

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Solution Summary

The fragments of human DNA that was generated by cutting with Ecor I and a plasmic DNA that was cut with Pst I is examined. DNA ligase for pieces of DNA are analyzed.

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The restriction enzyme ECOR I cuts DNA leaving 5' overhangs, Pst I leaves 3' overhangs after cutting DNA and SMA I leaves blunt ends on the fragments it produces. Indicate wheter DNA ligase can join the following pieces of DNA.

a) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was also cut with Ecor I .

b) a fragment of human DNA that was generated by cutting with Ecor I and a plasmid DNA that was cut with Pst I.

c) a fragment of human DNA that was generated by cutting with Sma I and a plasmid DNA that was also cut with Sma I.

Solution:

Restriction enzyme ECOR I cuts DNA, leaving 5' overhangs:

Recognition sequence is

5′---GAATTC

3′---CTTAAG

Note: Arrow marks indicate that the cut site (Target nucleotide sequence) for enzyme.

Restricted fragment will be.....

5′---G AATTC ---3′

3′---CTTAA G ...

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