G. Draw and label an in vitro cellular aging curve-Phase I, II, and III.
H. What are the typical conditions for growing human cells outside the body? Compare use of serum vs. defined medium.
I. Define a population doubling-in vitro. How many can you expect from a human, chicken, and mouse?
J. Explain pulse-labeled-nuclei, tritiated thymidine, and autoradiography.
K. Compare cell lines vs. cell strains.
M. What are the advantages/disadvantages to in vitro cell culturing as related to aging?
O. What is collagen? Why is this important to aging?