GRE Subject Question - Purification of a Protein

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• Protein Purification Questions

  303494 Protein Purification Questions Hey I am did a multiweek experiment doing a protein purification of Glutamate-Oxaloacetate Transaminase (GOT). I have a few questions about my experiment. 1.

• Protein Purity

  463348 Protein Purification How would you assess the purity of a protein? There are two good methods for assessing purity.

• Affinity Chromatography

  Well #2 is a sample of the serum before the purification procedure and shows that you do have quite a lot of the albumin in the serum, but there is also a lot of impurities, which you hope to get rid of by doing this affinity purification procedure.

• Contamination in protein purification

  Contamination in protein purification is a major issue and one that is not easy to eliminate.

• Why scientists purify proteins; principle of size exclusion

  Scientists in biotechnology must face the challenge of protein purification regularly. But the question arises, why do scientists need to take the pain?

• PI (isoelectric poin)

  you are purifying when developing a purification protocol and only use a range of pH in the column where the protein is active or at least does not denature).

• five step protocol

  This solution assists in interpreting the results of an enzyme purification.

• Peptide Sequencing

  GRE subject Question ________________________________________ You have isolated and purified a a small octapeptide protein and want to determine its primary structure. Deduce the sequence from the following experimental results.

• Null and Alternative Hypotheses

  It is the opposite of what the researcher sets out to conclude. Here the research question is whether there is a relationship between GRE scores and GPA- is there a correlation between them? The opposite of this is that there is no correlation.