Share
Explore BrainMass

Digestion Assay

Based on the following information how do I solve for 10x buffer, uls HOH, ul Enzyme, and Total?
Setup a digest that contains 10 ug of DNA/Sample, 3.5 ug/ul stock DNA (from experiment with 5ul of stock diluted with 995 H2O), 40 units of HAE III enzyme, and enzyme stock of 12 units/ul.

Sample ul DNA uls 10xBuffer uls HOH uls Enzyme Total ul
1 10ug/3.5 ug/ul

Please confirm DNA column, and demonstrate or provide formulas for the remaining columns.

Solution Preview

Hi there. Thanks for letting me work on your post. Here is my explanation:

If the ...

$2.19