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Site directed mutagenesis can be used to study ATP synthase

Conduction of protons by the F0 unit of ATP synthase is blocked by modification of a single side chain by dicyclohexylcarboiimide (DCCD). What are the most likely targets of action of this reagent? How might you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?

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DCCD is dicyclohexylcarbodiimide, a chemical reagent which reacts with acidic amino acid residues. This means that the most likely targets for this reagent are aspartic acid and glutamic acid residues, the two acidic amino acids that are part of proteins.

Using site directed mutagenesis means making mutations of the ...