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Net ATP Generation From 14CH3-COOH

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14CH3-COOH is fed to microorganisms during an experiment. Trace the 14C through the citric cycle. How many ATP molecules can be generated from 1 mol of this substance? (The conversion of acetate to acetyl-CoA required the consumption of 2 ATP.)

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Solution Summary

The expert calculates the ATP generated from an organism metabolizing 14CH3-COOH. The conversion of acetate to acetyl-CoA required is determined.

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We have to trace the label of 14CH3COOH through the Krebs cycle.
Here's how:

14CH3COOH -----> 14CH3CO-CoA

14CH3CO-CoA + OAA ----> citrate

Where is the label in citrate? Here is its structure:

14CH2-COOH
|
HO-C-COOH
|
CH2COOH

Then citrate gets dehydrated by aconitase to form cis-aconitate:

14CH2-COOH
|
C-COOH
||
CHCOOH

Then it gets hydrated to form isocitrate:

14CH2-COOH
...

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