14CH3-COOH is fed to microorganisms during an experiment. Trace the 14C through the citric cycle. How many ATP molecules can be generated from 1 mol of this substance? (The conversion of acetate to acetyl-CoA required the consumption of 2 ATP.)
We have to trace the label of 14CH3COOH through the Krebs cycle.
14CH3COOH -----> 14CH3CO-CoA
14CH3CO-CoA + OAA ----> citrate
Where is the label in citrate? Here is its structure:
Then citrate gets dehydrated by aconitase to form cis-aconitate:
Then it gets hydrated to form isocitrate:
Calculates ATP generated from an organism metabolizing 14CH3-COOH