Why are we most interested in using 6 base pair cutters?
Restriction enzymes are classified as endonucleases. Their biochemical activity is the hydrolysis ("digestion") of the phosphodiester backbone at specific sites in a DNA sequence. By "specific" we mean that an enzyme will only digest a DNA molecule after locating a particular sequence
<br>Enzymes with recognition sequences from 4 to 8 nucleotides in length each have uses in genetic engineering.
<br>(i.e. enzymes that have recognition sequences specified by six nucleotides) are good for day-to-day cloning work: You are using 6-cutters in the ...