I am recording intracellularly from the neocortex of a mouse. If I place my electrode on one set of pyramidal neurons (Layer 2) and add fenfluramine (a serotonin uptake blocker), whithin 5 minutes the neurons produce GREATER amount of action potentials. But when I place fenfluramine while recording from another set of pyramidal cells (Layer 5 for example), the neurons showed a DECREASE in AP's (action potentials) after 5 minutes of treatment. How is this possible for one drug to affect similar cells in completely different methods?
Please offer any feedback - if you feel that I haven't explained the question well enough, or if you aren't certain what I am looking for, please let me know. I am looking for help getting started on this and am hoping that you can provide some guidance.
Sorry for the delay in responding, but I have been experiencing some Microsoft Runtime Errors with internet explorer.
I found your posting to be very interesting. My approach to neuronal physiology is from how changes in neuronal physiology ultimately affect (or effect) the animal's behavior. Namely, my approach to this posting was in considering how and why one drug or chemical would affect similar cells differently, and why this sort of experimental manipulation may ...