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Acidic Fluid and Enzymatic Activity Experiment Example

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Describe an experiment in which you can test the effect of an acidic fluid on enzymatic activity that include one substrate, one enzyme, and one acidic fluid?

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Since this is only one credit, the answer will simply lead you to finding the correct methodology you will need to perform on your own. Remember to use the scientific method:
1. Design the experiment. Start with your (null) hypothesis and then provide a detailed account of the materials and methods used to conduct the experiment. Also include the ...

Solution Summary

The null hypothesis of this experiment is that acids have no effect on biological enzyme activities. The experiment is set up using the scientific method. Materials include hydrogen peroxide, plastic cups, and a source of catalase such as yeast or fresh meat.
Since catalase breaks down hydrogen peroxide into water and oxygen, the rate and extent of bubble formation are recorded as the results of the experiment.
An interpretation of the results as well as whether the null hypothesis is proven or not is encouraged.

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Using the scientific method for testing the effects of acidic fluid on enzymatic activity

Design and carry out an experiment in which you will test the effects of acidic fluid on enzymatic activity.

1. What question are you asking? Clearly state your hypothesis. Describe your IV and DV.
2. Design your experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis.
3. Conduct the experiment and record your results. Make a data table that summarizes your findings. You could include what you observed - "which samples showed bubbling?
4. Use your knowledge about enzymes to interpret your results. It may be necessary for you to refer to your textbook. What effect does the acid have on the enzyme?
5. Was your hypothesis supported? What is your conclusion?

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