Biology Homework Solutions
Problem
#1629

Understanding the structure and stability of base pairing in DNA. Predicting relative melting temperature (Tm) for DNA fragments.

Two duplex (ie. double stranded) DNA fragments are shown below.  Under identical conditions (ie. ionic strength, buffer composition, temperature, etc.), which double-stranded fragment would denature at the lower temperature?

Fragment A:

5' A-A-G-T-A-G-T-T-T-C-A 3'
3' T-T-C-A-T-C-A-A-A-G-T 5'

Fragment B:

5' G-C-A-G-G-T-C-G-C-C-T 3'
3' C-G-T-C-C-A-G-C-G-G-A 5'


Solution Summary

The solution provides a description of how to compare the melting temperature (also called denaturation temperature) of two or more duplex (double stranded) DNA sequences.  Hint:  when answering the question, take into account the number of hydrogen bonds between different base pairs

Solution
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Monica Tory, MSc - 3.8/5
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