Two duplex (ie. double stranded) DNA fragments are shown below. Under identical conditions (ie. ionic strength, buffer composition, temperature, etc.), which double-stranded fragment would denature at the lower temperature?
Fragment A:
5' A-A-G-T-A-G-T-T-T-C-A 3'
3' T-T-C-A-T-C-A-A-A-G-T 5'
Fragment B:
5' G-C-A-G-G-T-C-G-C-C-T 3'
3' C-G-T-C-C-A-G-C-G-G-A 5'
The solution provides a description of how to compare the melting temperature (also called denaturation temperature) of two or more duplex (double stranded) DNA sequences. Hint: when answering the question, take into account the number of hydrogen bonds between different base pairs