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Catalase Test Questions

We did the catalse test in class and I have been able to answer some questions but not all.
Questions:
1) Provide an example of reduction reaction performed by cells in which hydrogen is not added to the reduced compound. (I think it deals with hydrogen peroxide--> to water and oxygen)

2) Would a flase positive due to the reaction between the loop and hydrogen peroxide be due to a lack of specificy or sensitivity of the test system?

3) If a weakly catalase-positive organism itinitially gives a false-negative result and is not checked under the miicroscope, would the result be due to a lack of specificity or sensitivity of the test system?

4) Why is it advisable to perform this test on a known catalase-positive organism simultaneously with the organism you are checking?

5) What is the purpose of adding hydrogen peroxide to the uninoculated tube?

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Stephen Allen, MSc

Rating 4.9/5

Active since 2004

BSc, Dalhousie University, Halifax, Canada
MSc, Baylor College of Medicine, Houston, Texas
BA, Ambassador College, Big Sandy, Texas
Graduate Certificate, University of British Columbia, Vancouver, Canada

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