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You perform three microarray experiments in which you measure the mRNA levels in Rb mutant cell line (RB triangle) and in a control cell line (wild-type). The background subtracted. Normalized microarray data for three genes are given below. Please see the attached MS Word document for the chart. a. Floor the data to 50. ...continues
You perform DNA microarray experiments on cancer cells at different time points (0, 6, and 12 hours) following the addition of a novel anti-cancer drug. You obtain the following normalized and scaled microarray data for the genes Myc, p53, and Ras. Gene Name 0 hr 6 hr 12 hr Myc 1.0 ...continues
Fill-in each blank with the appropriate word(s) to complete the sentences. There are two major categories of cells. These categories are _______________ cells, which include bacteria, and __________________, which include fungi. ____________________ is the term that describes an organism's ability to maintain a stable intern ...continues
1. The gene pool is the sum total of all the ________ for every gene (locus) in a population. a) organs b) alleles c) reproductive aged individuals d) transcription factors e) mutations 2. A classification based on evolutionary relationships is called a ___________ classification a) genetic b) phylogenetic c) Mendelia ...continues
13. The gene pool is the sum total of all the ____ for every gene (locus) in a population. a) organs b) alleles c) reproductive aged individuals d) transcription factors e) mutations 14. A classification based on evolutionary relationships is called a _____ classification. a) genetic b) phylogenetic c) mendelian d ...continues
1. How do the base pairing relationships of DNA bases account for the uniform width of the double helix? 2. Look at Figure 13.13a-c on page 552 on the textbook. What enables the two DNA polymerase III molecules to act on replication as a unit (i.e., simultaneously replicating the leading and lagging strands) even though they ...continues
Attached in a .doc is a table guide to teach protein function through a nice internet tutorial
Using the document attached, use the Needleman-Wunsch dynamic programming method to align the following pair of sequences. (see attached) Use the unitary scoring method to score the initial alignment matrix. Write the appropriate score into each square in the matrix below. (see attached) Use the Needleman-Wunsch dynami ...continues
Here's a matrix value practice problem for writing sequence patterns.
1. You clone a double stranded DNA fragment from a pathogenic fungal stain. You suspect that the fragment contains a gene encoding a Seven Amino Acid Peptide. Here's one of the DNA strand fragments: 5' T G A G A T G C T T A G C T G C A A A C C T A T T T A A A C 3' A) What's the amino acid sequence of this peptide? B) ...continues
I need help with the following questions: Part 1: Determine the number of significant digits in each number and write out the specific significant digits. 1. 405000 2. 0.0098 3. 39.999999 4. 13.00 5. 80,000,089 6. 55,430.00 7. 0.000033 8. 620.03080 Part 2: Write the numbers below in scientific notation, incorpora ...continues
Please see the attachment for Table 1. Dissolved oxygen is oxygen that is trapped in a fluid, such as water. Since virtually every living organism requires oxygen to survive, it is a necessary component of water systems such as streams, lakes and rivers in order to support aquatic life. The dissolved oxygen is measured in uni ...continues
Multiple Choice Practice Problems 1) Exon prediction procedures (EX: hexmar frequency) works best in predicting which of the following exons? (A) 3' exons (B) 5' exons (C) Internal coding exons (D) Internal non-coding exons 2) Which of the following scoring matrices would work best for searching for divergent sequence ...continues
Plant and animal cells What is/are the functi0n(s) of 1. nucleus 2. mitochondrion 3. chloroplast 4. plasma membrane
I need assistance with the attached file please. 14. The lower the pH of a solution, the A. greater the number of oxygen atoms B. more acidic the solution C. less toxic the solution D. higher the OH" concentration . E. more basic the solution 15. Which of the following molecules are produced in light-dependent react ...continues
7. The building blocks of proteins are A. fatty acids B. amino acids C. nucleotides D. carbohydrates 18. The overall reaction of photosynthesis is A. H20 + CO2 --- O2 + C6H12O6 + H20 B. H2O + O2 --- CO2 + C6H12O6 + H2O C. H2O + CO2 + C6H12O6 --- 6O2 + CO2 + 6H2O D. H2O + NADH + CO2 --- O2 + C6H12O6 + H2O 9. Ferment ...continues
Are you familiar with the agar experiment? If so, please answer these questions.
See the attachment and answer the following: Determine the surface area and volume ratio for the three cubes. Which one if the most efficient in cellular morphology?
How does the movement of glucose across an epithelium involve both secondary active transport and facilitated diffusion?
Need help answering the following question. I have read my chapter and looked on the internet, but still cannot seem to answer the question. Explain how can a person be dead and 99% of his/her cells can be alive.
1. Perhaps you and/or your significant other is a carrier of a harmful recessive trait, such as cystic fibrosis. What would you do when considering having children? Would you hope for the best and ignore the situation or would you see a genetic counselor or what? 2. Choose a genetic disorder other than those discussed and te ...continues
Explain why asexually reproducing organisms are generally found in environments that do not change very much through time, while sexually reproducing organisms are very successful in environments that change dramatically through time.
Mexico is exhibiting growth similar to the population pyramid on the Mexico Male and Female diagram. Mexico is exhibiting what kind of growth? If Mexico enacted laws to limit the number of children per family (similar to China), how quickly would the country see a reduction in its population size? Why? How does this law fit into ...continues
Describe the role of double helix in complimentary base pairing in DNA replication. What does it mean when we say that the two strands of DNA in the double helix are antiparallel? What would the end of the double helix look like if the strands were parallel?
Hi, I need assistance understanding the following questions: Q1 - Choose one organism from the food web that fits into more than one trophic level and explain why and how this occurs. Q2- The introduction of non native species to Florida, such as the Melaleuca tree and the Australian Pine, create a disruption of the natura ...continues
Whether it was volcanic activity that spewed sulfur dioxide and ash into the atmosphere, or a giant meteor that crashed into the Earth blasting dust and debris into the sky, both theories of what caused the dinosaurs to become extinct share one critical similarity -- light from the sun was obliterated. The result of the months' ...continues
Can you please explain how to read the graph attached? I don't know what the use of rosiglitazone is for. What are the assumptions that come from the graph? (Answer provided in less than 100 words.)
I am reading up on a research paper Granted I have had very little sleep this last week as I am preparing for the final exam and prone to confusion At one point I loose understanding Can someone explain how using a luciferase reporter gene by being positioned next to the PPRE-containing fatty acyl CoA oxidase gene promoter ...continues
Please help me with the attached questions.
This solution will discuss how to elongate a piece of target DNA using the PCR process.
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