Picking the Best Inhibitor when Given Km, Kcat and Ki
Here are the following numbers I have calculated;
We were given a substrate to plot againist velocity and 3 enzymes to plot as well. From the data:
Enzyme A Ki= 2.575 | Kcat = 50 | Km= 3.33
Enzyme B Ki = 10.33 | Kcat = 16.65 | Km = 33.3
Enzyme C Ki= 1.00 | Kcat = 10 | Km = .2
I'm having trouble understanding which is the best inhibitor because:
A has the highest Kcat- best collision efficiency
C has the lowest Km - the highest affinity for its substrate
C has the lowest Ki- which means it binds the tightest.
The solution offers guidance and things to consider to pick the best inhibitor out of three when given their respective Kcat, Km and Ki values.
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