Expert Academic Help for Courses
Four Biochemistry Questions related to protein sequencing and protein structure.
1. You wish to use the Chou-Fasman method to predict the secondary structure of the following protein sequence: MCPQLNWKAYVKSAGARNLKGN a. Moving from left to right in the protein sequence, use the Chou-Fasman method to scan for the first group of six consecutive amino acids that are predicted to nucleate an alpha helix. D ...continues
Genetically modified organisms (GMO)
Pros and Cons of GMO Technology Pros: Genetically Modified Organisms(GMO) can benefit and contribute to medicine in many ways. For example, there are genetically modified microorganisms that can make human insulin to treat diabetic patients. (3) There are genetically modified bacteria that can be used to clean toxic fuel an ...continues
Briefly define the following terms: 1. stereospecific 2. denaturation 3. glycoprotein 4. assay 5. endergonic 6. active site 7. cytochrome 8. phosphofructokinase 9. specific activity 10. quaternary structure
Mitochondria and chloroplasts structure and function
Compare and contrast mitochondria and chloroplasts with respect to structure and function.
Describe the roles played by both transfer RNA and the ribosomes in protein synthesis.
Outline the Krebs cycle, and explain why it is central to eukaryotic catabolism
What are the ketone bodies? Why do they have that name, and how do they arise in metabolism? Explain why they accumulate under conditions of carbohydrate starvation or in diabetes.
The theory of recombinant DNA technology was enhanced and used in scientific research and now has practical medical applications in gene therapy. Within the last two decades, there have been major advances in treating genetic diseases that were once thought to be incurable. Discuss a current article where recombinant DNA technol ...continues
Viral Replication and Retroviral
Describe the process of viral replication; discuss both normal and retroviruses, and comment on the implications of this process for genetic engineering.
Outline the Embden-Meyerhof sequence and discuss the mechanism for regulating this pathway.
Chymotrypsin was able to hydrolyse the substrate N-benzoyl-L-tyrosine ethyl ester (BTEE) but not able to hydrolyse the substrate p-toluene sulphonyyl-L-arginine methyl ester (TAME). However the enzyme Trypsin was able to hydrolyse both TAME and BTEE substrates, and I was wondering why? And why/ what makes chymotrypsin so much ...continues
"The mechanism of action in which pyridoxine circumvents the human body is by being the precursor to pyridoxal, which functions in the metabolism of proteins, carbohydrates, and fats; pyridoxal also aids in the release of liver and muscle-stored glycogen and in the synthesis of GABA (within the central nervous system) and heme. ...continues
Ionisation of diacetylmorphine (heroin)
I have learn't that the pKa of diacetylmorphine is 7.6, close to physiological pH and hence will be mostly un-ionised in the blood. 1. How can diacetylmorphine be ionised in the blood and what will the effects of this be on the molecule in circulation? (I assume that it will be more soluble?) 2. When HCl is added to diace ...continues
Total number of ATP molecules produced from one molecule of maltose
Calculate total number of ATP molecules produced from one molecule of maltose. You will need to consider that first maltose will hydrolyze, then resulting glucose molecules will enter into glycolysis where ATP along with NADH molecules will be produced. Then resulting molecules of pyruvate enter into citric acid cycle and go all ...continues
Explain how this technique was and how it was applied to solve a biological question. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3312548/?tool=pubmed Besides labeling and identifying cells by their surface markers, what ate 2 other applications of flow cytometer techniques.
Calculations for Determining a Titer
The purpose of the experiment that took place was to determine the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay. I used a formula obtained from class mates to find the titer. I want to be sure this is the correct method for determining the titer. I attached my data, including the results from the plate ...continues
When considering "desirable" properties (chemical, biological, obtainability, etc.) for a bioweapon; can a mushroom toxin meet this criteria? Please give an example.
Determination of the Titer of B-D-Galactosidase Using the ELISA
The purpose of the experiment that took place was to determine the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay. given the results of the experiment, how do you determine the titer, and what do these results mean? The raw data (results obtained from the plate reader) are attached in the excel file. I fo ...continues
Graphs for ELISA practical: the conversion of ul/well to ng/well.
Hi, I am struggling to plot a graph in excel for my ELISA practical, the conversion of Amount of hCG (ul) to (ng) I have uploaded a doc. document with the Absorbance Results.
In this Nature journal article about the dog genome, how do I answer the following questions? What was the author's purpose? Why did the author decide to sequence the domestic dog's genome? What are 3 major conclusions that you learned from this paper? What future experiments did the authors propose, now that there is a d ...continues
This was a Investigation on Transcriptional Control of Genes in plants: Nitrate is assimilated in the leaves of higher plants by the enzymes nitrate reductase and nitrite reductase, the ammonia produced is incorporated into amino acids. NO3- Red NO2-Red NO3- NO2- NH4+ I Need help to convert values ...continues
Each gene 2 genes I have to find these answers to. Where is the polypeptide suppose to be located in the cell? Does your gene code for a transmembrane bound protein?why or why not Does your gene code for a signal peptide? Why or why not? Are these results consistent with your expectations? Why or why not?
Describe the E-value distribution are they small (close to zero) large (close to 1). Is the subject sequence from the top 2 BLAST hits from a genus and or a phylum that you would expect to be closely related to T. oceani? Are there any inconsistencies found in the T-coffee results, such as large polypeptide regions that ...continues
This lab provided a 100mM Glucose standard solution which you are required to dilute with water to give final concentrations of 0,0.4,0.8,1.2,1.6 and 2.0 mmol/L glucose in a final volume 0.5ml. You will then use those concentrations to generate a standard curve using a spectrophotometer in order to calculate the amount of glucos ...continues
Why do antibodies raised against a native protein sometimes fail to bind the corresponding denatured protein?
Restriction map for a circular plasmid.
I need to construct the possible restriction map for a circular plasmid. Please see data attached.
Describe various strategies for excretion of nitrogenous wastes employed in human and non-human chordates.
This solution discusses the requirements for nitrogen in living organisms, and how they differ between micro-organisms and higher life forms. Some organisms can use inorganic nitrogen, whereas others require organic nitrogen, and this is explained in detail in the solution.
DNA of the Human Body: Calculate the weight in grams of a double-helical DNA molecule stretching from the Earth to the moon (~320,000 km). The DNA double helix weighs about 1 x 10^-18 g per 1,000 nucleotide pairs; each base pair extends 3.4 Ã.... For an interesting comparison, your body contains about 0.5 g of DNA!
The endoplasmic reticulum: a) functions in internal transport of macromolecules. b) carries on cellular respiration. c) is the site of photosynthesis. d) is dispersed nuclear material of DNA and protein.